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Detection of Phytophthora nicotianae by PCR *
Author(s) -
Grote D.,
Olmos A.,
Kofoet A.,
Tuset J. J.,
Bertolini E.,
Cambra M.
Publication year - 2000
Publication title -
eppo bulletin
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.327
H-Index - 36
eISSN - 1365-2338
pISSN - 0250-8052
DOI - 10.1111/j.1365-2338.2000.tb00943.x
Subject(s) - phytophthora nicotianae , phytophthora , biology , polymerase chain reaction , pathogen , zoospore , botany , antiserum , mycelium , microbiology and biotechnology , genetics , gene , spore , antibody
Phytophthora nicotianae is an important soil‐borne pathogen. The classical determination based on morphological or physiological characters is time and labour‐consuming and is taxonomically unsure. Two specific primers designed from the spacer regions ITS1 and ITS2 were used to detect P. nicotianae by polymerase chain reaction (PCR). A PCR fragment of 737 bp was obtained from all P. nicotianae isolates assayed, but not from other Phytophthora spp. or other genera. Thus, the PCR characterization was species‐specific. Polyclonal antisera against mycelium and zoospores were used for comparison and were only genus‐specific.