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Detection of Ralstonia solanacearum from potato tissue by post‐enrichment TaqMan PCR *
Author(s) -
Weller S. A.,
Elphinstone J. G.,
Smith N. C.,
Stead D. E.
Publication year - 2000
Publication title -
eppo bulletin
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.327
H-Index - 36
eISSN - 1365-2338
pISSN - 0250-8052
DOI - 10.1111/j.1365-2338.2000.tb00915.x
Subject(s) - taqman , ralstonia solanacearum , biology , polymerase chain reaction , taq polymerase , real time polymerase chain reaction , pathogen , nuclease , population , microbiology and biotechnology , dna , gene , genetics , medicine , thermus aquaticus , environmental health
TaqMan polymerase chain reaction (PCR) exploits the 5′‐nuclease activity of Taq DNA polymerase in conjunction with fluorogenic DNA probes. A positive PCR reaction results in a measurable emission of fluorescence within 2 h. TaqMan assays for several plant‐pathogenic bacteria have been designed, including the potato brown rot pathogen Ralstonia solanacearum. Enrichment techniques designed to improve the detection of low pathogen populations in potato tissue are also suitable for use with TaqMan PCR. The quantitative aspect of TaqMan PCR enables relative population level differences between pre‐enriched and enriched samples to be demonstrated and thus cell viability to be evaluated.