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International programme on the serological detection of bacterial, fungal and viral pathogens of protein pea seeds 1
Author(s) -
LYONS N. F.,
TAYLOR J. D.,
ROBERTS S. J.,
MAURY Y.,
DUBY C.,
MASMOUDI K.,
FARISMOKAIESH S.,
CORBIÈRE R.,
MENDESPEREIRA E.,
SPIRE D.,
SAMSON R.,
MALANDRIN L.,
GRONDEAU C.,
FRANKEN A. A. J. M.,
FRAAIJE B. A.
Publication year - 1995
Publication title -
eppo bulletin
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.327
H-Index - 36
eISSN - 1365-2338
pISSN - 0250-8052
DOI - 10.1111/j.1365-2338.1995.tb01482.x
Subject(s) - biology , mycosphaerella , leaf spot , crop , agronomy , microbiology and biotechnology , horticulture
Arable (protein) peas are widely grown in northern Europe, specifically for use in animal feed. The main production areas (1 million ha) are France, UK and Denmark; The Netherlands is a major seed producer. There are a number of important seed‐borne pathogens, including bacterial blight ( Pseudomonas syringae pv. pisi ), leaf and pod spot ( Mycosphaerella pinodes ) and pea seed‐borne mosaic potyvirus (PSbMV), that cause crop losses. There is a need for improved rapid methods for the detection of these pathogens both to monitor the production of high‐quality seed and for quarantine purposes. Using knowledge of the specificity of antibodies raised to these organisms, techniques were developed for simultaneous detection of the pathogens from the same seed sample. Methods were developed that permitted the detection of 10 1 –10 2 bacterial cfu ml ‐1 in seed‐soak fluid, soluble antigen from seed with and without obvious fungal infection and the presence of PSbMV within the embryo of homogenized seed.