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A procedure for identification of polyphagous Liriomyza species using enzyme electrophoresis 1
Author(s) -
OUDMAN L.,
AUKEMA B.,
MENKEN S. B. J.,
ULENBERG S. A.
Publication year - 1995
Publication title -
eppo bulletin
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.327
H-Index - 36
eISSN - 1365-2338
pISSN - 0250-8052
DOI - 10.1111/j.1365-2338.1995.tb01477.x
Subject(s) - electrophoresis , biology , identification (biology) , species identification , cellulose acetate , enzyme , botany , cellulose , biochemistry , zoology
Liriomyza bryoniae, L. strigata, L. huidobrensis, L. trifolii and L. sativae are leafminers of great economic importance. Morphological identification is difficult and only possible in some life stages. Cellulose‐acetate gel electrophoresis provides a method for identification of all five species in each stage of the life cycle. The present paper describes the procedure for electrophoresis. The patterns of genetic variation of diagnostic enzymes and a biochemical key are presented. In addition, the electrophoretic patterns of the three most common parasitoids of Liriomyza are given.