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Diagnostic methods for the detection of faba bean necrotic yellows virus, a circular ssDNA virus 1
Author(s) -
KATUL L.,
VETTEN H. J.,
LESEMANN D.E.,
MAISS E.,
MAKKOUK K. M.
Publication year - 1995
Publication title -
eppo bulletin
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.327
H-Index - 36
eISSN - 1365-2338
pISSN - 0250-8052
DOI - 10.1111/j.1365-2338.1995.tb01474.x
Subject(s) - biology , immunoelectron microscopy , virus , virology , dot blot , aphid , plant virus , luteovirus , antiserum , western blot , microbiology and biotechnology , mottle , immunoassay , dna , antibody , botany , gene , genetics
Faba bean necrotic yellows virus (FBNYV), a circular ssDNA virus, infects a wide range of legume crops in West Asia and North Africa. Based on symptom development, host range and aphid transmission mode, FBNYV could not be differentiated from bean leaf roll luteovirus (BLRV). An antiserum prepared against purified FBNYV permitted specific detection of the virus and was used for its serological comparison with other ssDNA viruses and for the differentiation from BLRV by immunoelectron microscopy, ELISA, tissue blot immunoassay and Western blot analysis. Moreover, FBNYV DNA was 32 P‐labelled and used in dot‐blot hybridization experiments for virus detection in plant samples and further differentiation from BLRV.