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Use of PCR in the detection and characterization of geminiviruses 1
Author(s) -
BRIDDON R. W.,
MARKHAM P. G.
Publication year - 1995
Publication title -
eppo bulletin
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.327
H-Index - 36
eISSN - 1365-2338
pISSN - 0250-8052
DOI - 10.1111/j.1365-2338.1995.tb01472.x
Subject(s) - biology , geminiviridae , virology , polymerase chain reaction , plant virus , computational biology , virus , genetics , gene , begomovirus
Three methods utilizing PCR technology for the detection of members of the Geminiviridae are described. As well as being a tool useful for detection, the products of the diagnostic PCR reactions are suitable for further characterization of the viruses. Firstly, a pair of degenerate primers has been used to allow the detection of geminiviruses infecting dicotyledonous plants by PCR amplification. Secondly, a set of specific primers has been used which discriminate between the economically significant maize streak geminivirus and closely related viruses which infect mainly grasses. Finally, a PCR‐based method for the production of full‐length, infectious geminivirus clones is described. The advantages and disadvantages of PCR compared with other methods of detection and characterization are discussed.