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Use of tissue‐print immunoassay for the practical diagnosis of tomato spotted wilt tospovirus 1
Author(s) -
LOURO D.
Publication year - 1995
Publication title -
eppo bulletin
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.327
H-Index - 36
eISSN - 1365-2338
pISSN - 0250-8052
DOI - 10.1111/j.1365-2338.1995.tb01468.x
Subject(s) - immunoassay , tospovirus , biology , nitrocellulose , blot , tomato spotted wilt virus , antigen , plant virus , plant tissue , virology , antibody , virus , botany , immunology , biochemistry , membrane , gene
A tissue‐print immunoassay was developed for the detection of TSWV by direct printing of freshly cut tissue surfaces on nitrocellulose membranes. The adsorbed viral antigens were revealed by treating the blots with goat antibodies to biotin labelled with alkaline phosphatase. This indirect immunological method enables the reliable detection of TSWV in tissue blots from field samples of tomato, capsicum, chrysanthemum, gladiolus, hydrangea and oleander plants. In some cases, when ELISA tests gave negative or erratic results, TSWV antigens were readily detected in small cell clusters in the tissue blots. Considering the uneven distribution of TSWV in the plants, it is critical to sample from different tissues to select the best material for assaying. The tissue‐print immunoassay has the advantage of enabling specific diagnosis with the localization of the virus in the tissues. Thus, it might be a useful tool in tissue sampling for cytopathological studies. In addition, it is a simple, rapid and cheap method suitable for testing both small and large numbers of samples. Therefore, it is a technique which offers good prospects for critical diagnosis in quarantine cases and plant health certification programmes.