Premium
Development of a PCR‐based detection method for Xanthomonas campestris pv. translucens 1
Author(s) -
MAES M.,
GARBEVA P.
Publication year - 1995
Publication title -
eppo bulletin
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.327
H-Index - 36
eISSN - 1365-2338
pISSN - 0250-8052
DOI - 10.1111/j.1365-2338.1995.tb01459.x
Subject(s) - xanthomonas campestris , biology , bacteria , agarose gel electrophoresis , primer (cosmetics) , polymerase chain reaction , ribosomal dna , 16s ribosomal rna , botany , xanthomonas , microbiology and biotechnology , ribosomal rna , dna , genetics , gene , phylogenetics , chemistry , organic chemistry
Comparative sequence analysis of the ribosomal DNA of Xanthomonas campestris pv. translucens and of taxonomically related bacteria led to the development of a PCR‐mediated detection method for bacterial pathogens in the translucens/graminis complex of X. campestris. In these bacteria, a single 156‐bp fragment was synthesized. Other phytobacteria, taxonomically related to X.c. translucens or X.c. graminis or present on their plant hosts, did not show a PCR product on agarose gel electrophoresis. The detection system was used to screen bacterial populations in wheat and barley seed lots from American and Belgian production.