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Fatty acid analysis for the identification of Erwinia carotovora subsp. atroseptica and E. carotovora subsp. carotovora 1
Author(s) -
PERSSON P.,
SLETTEN A.
Publication year - 1995
Publication title -
eppo bulletin
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.327
H-Index - 36
eISSN - 1365-2338
pISSN - 0250-8052
DOI - 10.1111/j.1365-2338.1995.tb01452.x
Subject(s) - erwinia , bacteria , fatty acid , biology , enterobacteriaceae , microbiology and biotechnology , food science , chemistry , escherichia coli , biochemistry , gene , genetics
The pectolytic bacteria Erwinia carotovora subsp. atroseptica and E. carotovora subsp. carotovora were analysed by fatty acid profiling, using a gas chromatograph and a commercial software package for data processing. A fatty acid library was built from the analysis of 261 strains of the two bacteria from different sources and countries. Independently of source or geographical origin, 98% of the E.c. atroseptica strains and 97% of the E.c. carotovora strains were correctly identified. Analysis in two separate laboratories of identical strains gave highly reproducible results. Fatty acid profiling is a rapid, reliable and reproducible method for the identification and differentiation of these bacteria.
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