Premium
Profiling techniques for the identification and classification of plant pathogenic bacteria 1
Author(s) -
STEAD D. E.
Publication year - 1995
Publication title -
eppo bulletin
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.327
H-Index - 36
eISSN - 1365-2338
pISSN - 0250-8052
DOI - 10.1111/j.1365-2338.1995.tb01451.x
Subject(s) - profiling (computer programming) , dna profiling , computational biology , biology , bacterial taxonomy , restriction fragment length polymorphism , bacteria , computer science , genetics , genotype , gene , dna , 16s ribosomal rna , operating system
Profiling techniques are becoming widely used in the classification and identification of plant pathogenic bacteria. They are loosely defined as those techniques which produce a profile or fingerprint of a cellular component or its activity. They generally give much taxonomically useful information. They are relatively easily adapted for computerized comparisons of strains either for classification or for identification. The major genetic method is based on genomic fingerprinting or restriction fragment length polymorphism. Several phenetic chemotaxonomic methods are available, including protein profiling, fatty‐acid profiling and pyrolysis mass spectrometry. Nutritional profiling techniques are based on kits in which 20‐100 individual nutrients are included. All have their advantages and disadvantages and no single method should be used solely for classification or identification. Together with a few other well selected key tests, they have good potential as rapid, reproducible, low‐cost methods with high specificity. Several of these are now being commercially developed.