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Identification of races of Fusarium oxysporum f.sp. dianthi by DNA fingerprinting and vegetative compatibility 1
Author(s) -
WAALWIJK C.,
BAAYEN R. P.
Publication year - 1995
Publication title -
eppo bulletin
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.327
H-Index - 36
eISSN - 1365-2338
pISSN - 0250-8052
DOI - 10.1111/j.1365-2338.1995.tb01447.x
Subject(s) - carnation , biology , dna profiling , fusarium oxysporum , restriction fragment length polymorphism , botany , genetics , dna , polymerase chain reaction , gene
Knowledge on the geographic distribution of races of Fusarium oxysporum f.sp. dianthi and F. redolens f.sp. dianthi , causal agents of wilt disease of carnation, is of considerable importance to carnation breeders. The traditional way of identifying such races is by testing on differential cultivars but this is time‐consuming and not always reliable. In order to improve and accelerate the identification of new isolates, we have compared data from inoculation assays with several other methods. Sixty isolates, comprising all races of F.o. dianthi known to date, were analysed along with a number of isolates of F. redolens from carnation as well as F. oxysporum and F. redolens from other crops. The methods included VCG determination, where complementary mutants blocked in nitrate metabolism are assayed for their capability to form heterokaryons, analysis of RFLP patterns, using different genomic DNA fragments as probes, PCR analysis of the ITS region of the rDNA cistron, and RAPD analysis. The data obtained with these methods indicates that ITS‐PCR discriminates at species level, while RFLP analysis with two independent DNA probes differentiates between VCGs.