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Detection of benzimidazole resistance in Rhynchosporium secalis using allele‐specific oligonucleotide probes 1
Author(s) -
WHEELER I.,
KENDALL S.,
BUTTERS J.,
HOLLOMON D.
Publication year - 1995
Publication title -
eppo bulletin
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.327
H-Index - 36
eISSN - 1365-2338
pISSN - 0250-8052
DOI - 10.1111/j.1365-2338.1995.tb01446.x
Subject(s) - benzimidazole , biology , allele , oligonucleotide , oligomer restriction , fungicide , microbiology and biotechnology , genetics , dna , gene , chemistry , botany , organic chemistry
Benzimidazole fungicides are important mixture components in strategies to combat resistance in Rhynchosporium secalis as well as other plant pathogens. This strategy has recently been threatened by the detection of benzimidazole resistance in field strains of R. secalis. To evaluate the significance of this resistance, and to monitor its spread, a rapid and accurate method for identifying resistant alleles is needed. Consequently, we have developed a method for detecting benzimidazole resistance based on the hybridization of allele‐specific oligonucleotide (ASO) probes to PCR‐amplified DNA fragments.