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Identification of Fusarium oxysporum f.sp. cyclaminis by DNA fingerprinting and PCR 1
Author(s) -
WOUDT L. P.
Publication year - 1995
Publication title -
eppo bulletin
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.327
H-Index - 36
eISSN - 1365-2338
pISSN - 0250-8052
DOI - 10.1111/j.1365-2338.1995.tb01445.x
Subject(s) - fusarium oxysporum , biology , dna profiling , genomic dna , dna , pathogen , identification (biology) , microbiology and biotechnology , polymerase chain reaction , genetics , gene , botany
A set of 55 isolates of the wilt pathogen Fusarium oxysporum f.sp. cyclaminis was separated into three groups by DNA fingerprinting. A common genomic DNA fragment was identified in all isolates. This fragment was cloned and found to consist of subfragments which are also present in non‐pathogenic isolates of F. oxysporum obtained from cyclamen plant material, but not similarly linked. PCR primers derived from these subfragments proved to be effective tools for identification and detection of F.o. cyclaminis.