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A monoclonal antibody immunoassay for the detection of Nectria galligena in apple fruit and woody tissues 1
Author(s) -
DEWEY F. M.,
LI R.,
SWINBURNE T.
Publication year - 1995
Publication title -
eppo bulletin
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.327
H-Index - 36
eISSN - 1365-2338
pISSN - 0250-8052
DOI - 10.1111/j.1365-2338.1995.tb01439.x
Subject(s) - polyclonal antibodies , monoclonal antibody , antiserum , immunofluorescence , microbiology and biotechnology , biology , antibody , immunoassay , monoclonal , cell culture , antigen , pathogen , immunization , virology , immunology , genetics
Relatively rapid, highly specific monoclonal antibody‐based enzyme‐linked immunosorbent and immunofluorescent assays have been developed to detect and visualize Nectria galligena in woody tissue. Hybridoma cell lines were raised by co‐immunization of mice with extracts from freeze‐dried cultures of N. galligena and murine polyclonal antiserum to a related pathogen, N. haematococca. This method appeared to reduce the number of hybridoma cell lines that secreted cross‐reactive antibodies. From two fusions, ten cell lines that recognized N. galligena and not N. cinnabarina were raised. Hybridoma supernatants were screened by ELISA using antigen‐coated wells. Monoclonal antibodies from one cell line, NG‐IE4, proved useful in the detection of the pathogen in woody tissue by ELISA and antibodies from another cell line, NG‐DF11, have been used to immunolocalize the fungus in woody tissue by immunofluorescence. Further applications are ongoing.