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Immunodetection of Botrytis allii , the causal agent of neck rot of onion bulbs 1
Author(s) -
LINFIELD C. A.,
LYONS N. F.,
KENNY S. R.
Publication year - 1995
Publication title -
eppo bulletin
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.327
H-Index - 36
eISSN - 1365-2338
pISSN - 0250-8052
DOI - 10.1111/j.1365-2338.1995.tb01436.x
Subject(s) - antiserum , polyclonal antibodies , biology , botrytis cinerea , botrytis , pathogen , microbiology and biotechnology , sensu stricto , antigen , virology , botany , immunology , zoology
Polyclonal antisera for the immunological detection of Botrytis allii were raised from cell wall, cytoplasmic and surface plate washings of two isolates of the pathogen. These antisera were used to develop an indirect ELISA for the detection of latent B. allii infection in stored onion bulbs. The antisera did not react with healthy onion tissue and showed only minimal recognition of fungi from other genera. Some cross reactivity was observed with B. cinerea but not with other Botrytis species tested. In many instances following infection, fungal antigen could be detected earlier by ELISA than by direct plating. In tests on bulbs from a commercial store, the level of B. allii infection was found to be 3.3% by both ELISA and direct plating, but this result was achieved 7 days sooner by ELISA.