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The use of polymerase chain reaction to detect latent infection of Clavibacter michiganensis subsp. michiganensis in tomato seedlings
Author(s) -
GHEDINI R.,
FIORE N.
Publication year - 1995
Publication title -
eppo bulletin
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.327
H-Index - 36
eISSN - 1365-2338
pISSN - 0250-8052
DOI - 10.1111/j.1365-2338.1995.tb00578.x
Subject(s) - clavibacter michiganensis , polymerase chain reaction , biology , seedling , microbiology and biotechnology , horticulture , genetics , pathogen , gene
A method was developed for routine analysis to detect latent infection of Clavibacter michiganensis subsp. michiganensis in samples from tomato seedling lots, before transplant, using polymerase chain reaction. In samples of 300 stem segments 1 cm long, the sensitivity threshold of the method was estimated at around 1.1 × 10 3 corynebacteria or 0.33% of latently infected stems. This method was shown to have a good specificity.