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Production of plants of Nicotiana benthamiana and Prunus domestica transgenic for plum pox potyvirus coat protein, and demonstration of PPV resistance in transformed N. benthamiana 1
Author(s) -
RAVELONANDRO M.,
SCORZA R.,
CALLAHAN A.,
CORDTS J.,
MONSION M.,
FUCHS M.,
DELBOS R.,
BACHELIER J.,
GONSALVES D.,
DUNEZ J.
Publication year - 1994
Publication title -
eppo bulletin
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.327
H-Index - 36
eISSN - 1365-2338
pISSN - 0250-8052
DOI - 10.1111/j.1365-2338.1994.tb01087.x
Subject(s) - nicotiana benthamiana , biology , potyvirus , agrobacterium tumefaciens , inoculation , genetically modified crops , virology , transgene , prunus , potyviridae , plant virus , virus , gene , botany , horticulture , genetics
Leaf discs of Nicotiana benthamiana plants were transformed with Agrobacterium tumefaciens and transgenic plants expressing plum pox potyvirus (PPV) coat protein (CP) were generated. Homozygous R 2 progeny from these plants were inoculated with PPV. Plants were scored for the appearance of symptoms and tested for infection by DAS‐ELISA. Various levels of resistance were obtained after an initial stage in which PPV was able to multiply in all the transgenic plants. Within 2–3 weeks after inoculation, the transgenic resistant plants fully recovered from virus infection. Conversely, control and susceptible transgenic lines developed severe symptoms and high virus titres. Prunus domestica (plum) was transformed by inoculating hypocotyl slices with A. tumefaciens containing a binary plasmid which included the NPTII, GUS, and PPV CP genes within its T‐DNA region. Transgenic shoots were rooted and established in the glasshouse. Analysis of selected transformants by PCR showed that the engineered foreign genes had been integrated, including that for PPV CP. Histological assays on young leaves of these putative transformants gave a positive reaction. This suggests that all genes transferred are expressed in these transformed plums.