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Approaches to pathogen‐mediated resistance breeding against plum pox potyvirus in stone‐fruit trees 1
Author(s) -
CÂMARA MACHADO A.,
KNAPP E.,
PÜHRINGER H.,
SEIFERT G.,
HANZER V.,
WEISS H.,
KATINGER H.,
CÂMARA MACHADO M. LAIMER
Publication year - 1994
Publication title -
eppo bulletin
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.327
H-Index - 36
eISSN - 1365-2338
pISSN - 0250-8052
DOI - 10.1111/j.1365-2338.1994.tb01085.x
Subject(s) - biology , potyvirus , agrobacterium tumefaciens , gene , transformation (genetics) , agrobacterium , potyviridae , botany , horticulture , virology , genetics , plant virus , virus
In a programme for developing systems which allow the transfer of foreign genes into apricot cultivars, we have tested cotyledons of immature embryos, somatic embryos and leaf discs. Apricot plants have been transformed, and then regenerated, with Agrobacterium tumefaciens strain LBA 4404 containing various binary plasmids: pBinGUSint, carrying the marker gene β‐glucuronidase (GUS), and pBinPPVm, carrying the coat‐protein gene of plum pox potyvirus (PPV). The marker gene GUS was used for visual evaluation of the efficiency of the transformation system. The coat‐protein gene was used in the hope of introducing coat protein‐mediated resistance to one of the most important stone‐fruit pathogens in Europe and the Mediterranean area.