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Cross reactions of an antiserum to plum pox potyvirus 1
Author(s) -
JAMES D.,
THOMPSON D. A.,
GODKIN S. E.
Publication year - 1994
Publication title -
eppo bulletin
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.327
H-Index - 36
eISSN - 1365-2338
pISSN - 0250-8052
DOI - 10.1111/j.1365-2338.1994.tb01074.x
Subject(s) - potyvirus , antiserum , virology , herbaceous plant , biology , prunus , pox virus , western blot , potyviridae , plant virus , microbiology and biotechnology , virus , botany , antibody , gene , genetics
In the early spring of 1992, plum pox‐like viruses (PPLVs) were detected by standard ELISA in some Prunus species. The isolates reacted positively with plum pox potyvirus (PPV) antisera in immunosorbent electron microscopy and Western blot analysis. In Western blot analyses, bands associated with the coat protein subunits of the PPLVs were 48–56 kDa, whereas bands associated with the coat protein subunits of known PPV isolates were 32–37 kDa in size. Also, the PPLVs differed from known PPV isolates in their symptoms on woody and herbaceous indicators, and in their herbaceous host range. None of these PPLVs appears to be an isolate of PPV.