Premium
Detection methods for Xanithomonas campestris pv. graminis on forage grasses 1
Author(s) -
SLETTEN A.
Publication year - 1989
Publication title -
eppo bulletin
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.327
H-Index - 36
eISSN - 1365-2338
pISSN - 0250-8052
DOI - 10.1111/j.1365-2338.1989.tb00130.x
Subject(s) - xanthomonas campestris , bacterial wilt , biology , forage , bacteria , phleum , botany , pathogen , isolation (microbiology) , host (biology) , lolium , seedling , poaceae , microbiology and biotechnology , ecology , genetics
In Norway seven different grass species were found to be attacked by Xanthomonas campestris pv. graminis (bacterial wilt of forage grasses). Among these, Phleum pratense is the most susceptible and common host. Considerable damage may occur after long periods of hot and dry weather, while in cool and wet periods hardly any diseased plants are found. The pathogen may be identified by isolation and biochemical tests. Specific antibodies used in immunofluorescence and ELISA had a high degree of sensitivity and specificity against the target bacterium. The two methods were used for screening pure cultures and detecting bacteria directly in plant tissue extracts. Their application revealed the presence of low numbers of bacteria in symptomless plants and a discontinuous distribution within the plant.