Premium
A detection method for Pseudomonas solanacearum in symptomless potato tubers and some data on its sensitivity and specificity
Author(s) -
JANSE J. D.
Publication year - 1988
Publication title -
eppo bulletin
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.327
H-Index - 36
eISSN - 1365-2338
pISSN - 0250-8052
DOI - 10.1111/j.1365-2338.1988.tb00385.x
Subject(s) - polyclonal antibodies , bacterial wilt , antiserum , pseudomonas , ralstonia solanacearum , microbiology and biotechnology , pathogenicity , biology , bacteria , chromatography , antibody , horticulture , chemistry , pathogen , immunology , genetics
A detection method is described for latent infections of Pseudomonas solanacearum race 3 in potato. This method is based on extraction of 200 heel ends of potato, followed by screening with an indirect antibody stain (IFAS) and (in IFAS‐positive cases) a pathogenicity test on tomato (PT). Using the method in some sensitivity and specificity tests with more than 600 samples it appears that: (a) its detection level is 10 4 cells ml ‐1 in IFAS and 10 2 — 10 4 cells ml ‐1 in PT; (b) per cent recovery is 0.1–10% (10% at lower contamination levels); (c) false‐positive samples due to cross‐reacting bacteria in IFAS were limited to only 2–3%, using two polyclonal antisera against whole cells of P. solanacearum. The merits of the method in comparison with others (ELISA, selective media) are discussed.