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Practical use of ELISA to detect cauliflower mosaic virus in cauliflower 1
Author(s) -
KERLAN C.,
MEVEL S.
Publication year - 1987
Publication title -
eppo bulletin
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.327
H-Index - 36
eISSN - 1365-2338
pISSN - 0250-8052
DOI - 10.1111/j.1365-2338.1987.tb00017.x
Subject(s) - turnip mosaic virus , cauliflower mosaic virus , strain (injury) , antiserum , virology , biology , virus , serology , plant virus , horticulture , antibody , biochemistry , potyvirus , genetically modified crops , transgene , anatomy , gene , immunology
An antiserum was prepared against cauliflower mosaic virus strain S. The specificity of the reagents (IgG labelled with alkaline phosphatase) allowed the use of ELISA test (standard method) for virus detection, directly from cauliflower plants. Triton and urea had to be added to the homogenizing phosphate buffer. Labelled antibodies from strain S were also reactive ‐ with high optical densities ‐ against strain PV‐45 and Cabb‐BJI, but a distant relationship appeared with strain CM4‐184. Two thousand samples picked in the field were analysed and some results showed that the virus could be latent in the plant. A good correlation was observed between ELISA and biological indexing results. However, the serological diagnosis was more precise when cauliflowers were doubly infected by cauliflower mosaic virus and turnip mosaic virus.