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Problems associated with potyviruses in potato certification‐field inspection and serological testing 1
Author(s) -
JONES R. A. C.
Publication year - 1987
Publication title -
eppo bulletin
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.327
H-Index - 36
eISSN - 1365-2338
pISSN - 0250-8052
DOI - 10.1111/j.1365-2338.1987.tb00008.x
Subject(s) - potato virus y , potyvirus , virology , serology , potyviridae , cultivar , strain (injury) , biology , virus , veterinary medicine , horticulture , medicine , plant virus , antibody , immunology , anatomy
In UK, the tobacco veinal necrosis strain of potato virus Y (PVY N ), potato virus A (PVA) and potato virus V (PVV) each occur in the field only in limited ranges of potato cultivars in which they mostly cause mild symptoms or even symptomless infection; little is known about incidence of strain C of PVY (PVY C ). The ordinary strain of PVY (PVY°), however, is widespread causing symptoms ranging in severity from very severe through to very mild, depending on cultivar sensitivity/tolerance. During field inspections, very mild potyvirus symptoms may be missed, so inspectors are trained to be particularly vigilant when examining problem cultivars which react in this way. PVA is almost invariably treated, along with PVX, as a mild kind of virus infection, but infections with PVY°, PVY N and PVV are treated as severe with stricter tolerances being applied for them (especially for PVY N ) regardless of symptom severity. Wide variation within the same cultivar in the behaviour of variants within the PVY° strain group also sometimes causes difficulties in interpretation at inspection. To detect PVY, PVA and PVV in routine serological testing on potato certification samples, it is necessary to employ specific antisera to each of them. PVY N ‐specific monoclonal antibodies can be used in ELISA to distinguish PVY N from PVY°.