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Interpretation of the EC method for the detection of latent Corynebacterium sepedonicum infections in potato 1
Author(s) -
JANSE J. D.,
VAERENBERGH J.
Publication year - 1987
Publication title -
eppo bulletin
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.327
H-Index - 36
eISSN - 1365-2338
pISSN - 0250-8052
DOI - 10.1111/j.1365-2338.1987.tb00001.x
Subject(s) - corynebacterium , pathogenicity , maceration (sewage) , biology , horticulture , microbiology and biotechnology , food science , bacteria , genetics , materials science , composite material
The EC method for the detection of latent ring‐rot infections ( Corynebacterium sepedonicum ) consists essentially of an indirect immunofluorescence test and a pathogenicity test on eggplant (EP). When interpreting the results of this method, care should be taken that: (a) eggplants are grown at 21°C. At 28–29°C the detection level of the EP was increased 10 2 to 10 3 ‐fold to 10 5 to 10 6 cells ml ‐1 and latent infections (which may go unnoticed) occurred with 10 4 to 10 5 cells ml ‐1 ; (b) reisolations are made from symptomless eggplants grown at the optimum temperature (21°C) as latent infections can also occur in these plants at concentrations of 10 2 to 10 3 cells ml ‐1 ; (c) potato tuber extracts are tested without freezing or stored freeze‐dried or in a proper protective agent. Freezing at ‐20°C in maceration buffer of cells of C. sepedonicum and Pseudomonas solanacearum (the latter was included for comparison) reduced their numbers by 90–98% after one day. This could easily cause viable cell numbers to drop below the detection level of the pathogenicity test.