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Detection of Human Polyomavirus Dna In Papanicolaou Stained Smears of Urinary Sediment By In Situ Hybridization
Author(s) -
IWA N.,
KOBAYASHI T. K.,
YUTANI C.
Publication year - 1991
Publication title -
cytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 48
eISSN - 1365-2303
pISSN - 0956-5507
DOI - 10.1111/j.1365-2303.1991.tb00396.x
Subject(s) - papanicolaou stain , in situ hybridization , urinary sediment , urinary system , cytology , in situ , medicine , nucleic acid thermodynamics , hybridization probe , biotinylation , dna , pathology , virology , biology , microbiology and biotechnology , cancer , chemistry , cervical cancer , anatomy , biochemistry , gene expression , genetics , organic chemistry , base sequence , gene
Papanicolaou stained smears of urinary sediment containing inclusion bearing urothelial cells suggestive of human polyomavirus infection were destained and reprocessed for in situ hybridization using a biotinylated probe for human polyomavirus DNA. Seven slides were processed in this way. A hybridization signal for viral DNA was noted in each case, even in smears that had previously been stored for 11 years. This simple and rapid non‐radioactive detection system is a valuable supplement to routine urinary cytology for the definitive diagnosis of this virus infection.