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Elastic scattering spectroscopy in the head & neck
Author(s) -
Upile T.,
Jerjes W.,
Howard D.,
Michaels L.,
Hopper C.,
Wright A.
Publication year - 2006
Publication title -
clinical otolaryngology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.914
H-Index - 68
eISSN - 1749-4486
pISSN - 1749-4478
DOI - 10.1111/j.1365-2273.2006.01341_14.x
Subject(s) - medicine , biopsy , gold standard (test) , in vivo , head and neck , ex vivo , spectroscopy , leukoplakia , pathology , nuclear medicine , radiology , surgery , cancer , quantum mechanics , biology , physics , microbiology and biotechnology
Objectives. To develop the a technique of optical biopsy of oral dysplastic lesions that will provide real‐time diagnosis, allow in situ monitoring, and which will be feasible in the clinical environment especially for patient follow‐up. Method. Ethics approval was granted. Ex‐vivo specimens were evaluated to determine the optimal protocol before use in vivo . 75 spectra were obtained from 25 patients with oral leukoplakia. The spectral readings were mapped, and the area was then biopsied. Photographic images were co‐registered with the histological specimen. The sensitivity and specificity of the technique was determined compared to the gold standard of histo‐pathological examination of paraffin sections. Results. The sensitivity and specificity in determining dysplastic from benign conditions were 70% and 78.6% respectively at 580 nm. Conclusions. The use of Elastic Scattering Spectroscopy may aid the differentiation of normal and dysplastic mucosal lesions in the head & neck. References 1 Chung, A., Karlan, S., Lindsley, E., et al. (2006) In vivo cytometry: a spectrum of possibilities. Cytometry A . 69 , 142–146 2 Dhar, A., Johnson, KS., Novelli, MR., et al. (2006) Elastic scattering spectroscopy for the diagnosis of colonic lesions: initial results of a novel optical biopsy technique. Gastrointest Endosc. 63 , 257–261