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Thiazolidinediones and antiblastics in primary human anaplastic thyroid cancer cells
Author(s) -
Antonelli Alessandro,
Ferrari Silvia Martina,
Fallahi Poupak,
Berti Piero,
Materazzi Gabriele,
Minuto Michele,
Giannini Riccardo,
Marchetti Ivo,
Barani Lucio,
Basolo Fulvio,
Ferrannini Ele,
Miccoli Paolo
Publication year - 2009
Publication title -
clinical endocrinology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.055
H-Index - 147
eISSN - 1365-2265
pISSN - 0300-0664
DOI - 10.1111/j.1365-2265.2008.03415.x
Subject(s) - rosiglitazone , pioglitazone , anaplastic thyroid cancer , medicine , endocrinology , bleomycin , anaplastic carcinoma , cell growth , thyroid , thyroid cancer , chemistry , chemotherapy , receptor , type 2 diabetes , biochemistry , diabetes mellitus
Summary Objective No study has evaluated the antiproliferative effects of thiazolidinediones and antiblastics in ‘primary cultured human anaplastic thyroid cancer cells’. Design Primary anaplastic cells proliferation was evaluated after incubation with increasing concentrations of rosiglitazone or pioglitazone or antiblastics (bleomycin, cisplatin, gemcitabine) by a proliferation assay (WST‐1‐tetrazolium reaction) and cell counting. Measurements and results A reduction of proliferation by thiazolidinediones at 1 h (from the start of tetrazolium reaction) [of 11% and 25%, with rosiglitazone, 10 or 20 ( P = 0·0001) µM, respectively; of 7% and 17%, with pioglitazone, 10 or 20 ( P = 0·0125) µM, respectively], and at 2 h [of 14% and 24%, with rosiglitazone, 10 ( P = 0·0043) or 20 ( P < 0·0001) µM, respectively; of 9% and 21%, with pioglitazone, 10 ( P = 0·0397) or 20 ( P = 0·0001) µM, respectively] was shown. No significant thiazolidinediones effect was observed in normal thyroid follicular cells. Bleomycin, cisplatin and gemcitabine significantly ( P < 0·0001) inhibited (> 50%) anaplastic cells proliferation. Cell counting confirmed the above mentioned results. Inhibition of proliferation was similar in tumours with or without V600E BRAF mutation, both for thiazolidinediones and antiblastics. Conclusions Thiazolidinediones exert an antiproliferative effect in primary cultured human anaplastic carcinoma cells in vitro , such as antiblastics.