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Aberrant adrenal sensitivity to vasopressin in adrenal tumours associated with subclinical or overt autonomous hypercortisolism: is this explained by an overexpression of vasopressin receptors?
Author(s) -
Joubert Michael,
Louiset Estelle,
Rego JeanLuc Do,
Contesse Vincent,
Kong Ling Chun,
Benhaim Annie,
Mittre Hervé,
Lefebvre Hervé,
Reznik Yves
Publication year - 2008
Publication title -
clinical endocrinology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.055
H-Index - 147
eISSN - 1365-2265
pISSN - 0300-0664
DOI - 10.1111/j.1365-2265.2007.03106.x
Subject(s) - vasopressin , endocrinology , medicine , vasopressin receptor , adrenocortical carcinoma , arginine vasopressin receptor 1b , arginine vasopressin receptor 2 , receptor , biology , in vivo , adrenal gland , antagonist , microbiology and biotechnology
Summary Objective Abnormal responsiveness to arginine vasopressin (AVP) was previously observed in cortisol‐producing adrenocortical tumours but the mechanism remains unclear. The aim of this study was to characterize the effect of AVP on cortisol secretion from adrenocortical tumours compared to normal human adrenal gland. Design A multicentre study based on pharmacological, molecular and immunohistochemical experiments performed in adenomatous and normal adrenal tissues. Patients Twenty patients with adrenocortical adenomas and subclinical Cushing's syndrome (SCCS) or Cushing's syndrome (CS) were compared to six control normal subjects. Measurements In vivo and in vitro cortisol response to vasopressin, vasopressin receptor subtype mRNA measurement by real‐time polymerase chain reaction (RT‐PCR), immunohistochemical localization of AVP and its V1a receptor in tumour and normal adrenal tissues. Results Terlipressin in vivo enhanced cortisol plasma levels in 17/20 SCCS and 3/6 CS but in none of the control subjects. In vitro cortisol response to AVP was observed in nine tumours studied, with enhanced efficacy and/or potency of AVP in three SCCS tumours compared to normal tissues. AVP receptor subtype mRNA levels were similar in SCCS, CS cells and normal adrenal cells. Some SCCS tumour steroidogenic cells showed AVP and V1a receptor immunoreactivity. Conclusions SCCS and CS adrenocortical tumours often exhibit in vivo and in vitro hyper‐responsiveness to AVP, which is not related to vasopressin receptor overexpression, but may be explained by more efficient coupling pathways or by the indirect action of AVP through an autocrine/paracrine mechanism.