Premium
Effects of free fatty acids, growth hormone and growth hormone receptor blockade on serum ghrelin levels in humans
Author(s) -
Gormsen Lars C.,
Nielsen Charlotte,
Gjedsted Jakob,
Gjedde Signe,
Vestergaard Esben Thyssen,
Christiansen Jens S.,
Jørgensen Jens Otto,
Møller Niels
Publication year - 2007
Publication title -
clinical endocrinology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.055
H-Index - 147
eISSN - 1365-2265
pISSN - 0300-0664
DOI - 10.1111/j.1365-2265.2007.02786.x
Subject(s) - ghrelin , medicine , endocrinology , insulin , growth hormone receptor , blockade , pegvisomant , pancreatic hormone , chemistry , hormone , receptor , insulin resistance , growth hormone , acromegaly
Summary Background Circulating ghrelin levels are reported to be suppressed by insulin, GH and free fatty acids (FFAs). However, insulin, GH and FFA levels are all interdependent, and it is therefore difficult to delineate their independent effects on ghrelin secretion. Objective To isolate and define the impact of GH, GH receptor (GHR) blockade and intravenous FFA infusion on total circulating ghrelin levels during a hyperinsulinaemic glucose clamp with identical insulin levels. Design In a randomized design, eight healthy males each underwent an 8‐h hyperinsulinaemic glucose clamp on four occasions together with either: (1) control (saline), (2) intravenous FFA infusion (intralipid/heparin infusion 4 h), (3) a GH bolus (0·5 mg i.v.) or (4) GHR blockade (pegvisomant, 30 mg s.c.). Results Hyperinsulinaemia per se resulted in a decrease in ghrelin concentrations of about 15%. During FFA exposure, ghrelin levels were suppressed by about 22% when compared with saline [area under the curve (AUC) ghrelin0−240 122·7 ± 10·9 vs. 97·6 ± 13·4 pg/ml/min, P = 0·001], followed by a rebound increase upon discontinuation of the infusion. Furthermore, average ghrelin concentration (AUC ghrelin ) was significantly inversely correlated to average FFA levels (AUC FFA ) ( r = − 0·33, P < 0·05). Neither GH administration nor GHR blockade resulted in significant alterations in total ghrelin levels in the presence of unaltered insulin and FFA levels. Conclusions Elevation of FFAs by means of an intravenous infusion acutely suppresses ghrelin levels, whereas GH administration and GHR blockade have no detectable effect on ghrelin concentration when insulin and FFA levels are kept fixed.