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Breast cyst fluids increase the proliferation of breast cell lines in correlation with their hormone and growth factor concentration
Author(s) -
Enriori Pablo J.,
Vázquez Stella Maris,
Chiauzzi Violeta,
Pérez Cecilia,
Fischer Carlos R.,
Gori Jorge R.,
Etkin Alberto E.,
Charreau Eduardo,
Calandra Ricardo S.,
Lüthy Isabel A.
Publication year - 2006
Publication title -
clinical endocrinology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.055
H-Index - 147
eISSN - 1365-2265
pISSN - 0300-0664
DOI - 10.1111/j.1365-2265.2005.02408.x
Subject(s) - endocrinology , medicine , epidermal growth factor , mcf 7 , transforming growth factor , growth factor , cell growth , cyst , biology , mammary gland , cell culture , thymidine , chemistry , breast cancer , cancer , receptor , human breast , pathology , biochemistry , in vitro , genetics
Summary Objective and design Gross cystic disease (GCD) of the breast is reported to occur in 7% of women in the developed world and, although not premalignant, is thought to be associated with an increased risk of breast cancer. Hormone and growth factor concentration levels were measured in breast cyst fluid (BCF) to correlate them with their mitogenic activity in tumour (MCF‐7) or nontransformed (MCF‐10A) cells. Results Oestradiol (E 2 ), oestrone (E 1 ), E 2 ‐sulfate (E 2 ‐S), E 1 ‐sulfate (E 1 ‐S) and epidermal growth factor (EGF) concentrations were, as expected, significantly higher in type I than in type II cysts, while transforming growth factor‐beta 2 (TGF‐β2) showed higher levels in type II cysts. Fifty per cent of the BCF samples stimulated [ 3 H]‐thymidine incorporation into MCF‐7 cells while 34·5% inhibited this parameter. In MCF‐10A cells, most BCF samples were stimulatory (85%). E 2 , E 1 and EGF concentrations in BCF samples correlated significantly and positively with cell proliferation in MCF‐7 cells, whereas a significant negative correlation was found for TGF‐β2. In MCF‐10A cells, only E 2 ‐S and E 1 ‐S exhibited significant positive correlation, whereas a significant negative correlation was found for TGF‐β2. Progesterone (Pg), E 2 and EGF incubated under the same conditions had a stimulatory effect on [ 3 H]‐thymidine incorporation into MCF‐7 cells, whereas TGF‐β2 inhibited this parameter. Pg, E 2 , E 1 and EGF significantly stimulated this parameter in MCF‐10A cells. Conclusions The stimulatory action of BCF on cell proliferation in a model of human breast epithelial cells could partly explain the increased incidence of breast cancer in cyst‐bearing women.