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Seladin‐1/DHCR24 expression in normal ovary, ovarian epithelial and granulosa tumours
Author(s) -
Fuller Peter J.,
Alexiadis Maria,
Jobling Tom,
McNeilage Jane
Publication year - 2005
Publication title -
clinical endocrinology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.055
H-Index - 147
eISSN - 1365-2265
pISSN - 0300-0664
DOI - 10.1111/j.1365-2265.2005.02308.x
Subject(s) - ovary , mucinous cystadenocarcinoma , biology , endocrinology , serous cystadenocarcinoma , medicine , granulosa cell , western blot , serous fluid , ovarian cancer , gene , cancer , biochemistry
Summary Objective The human DIMINUTO/DWARF1 homolog seladin‐1/DHCR24 has been recently reported to be up‐regulated in adrenocortical adenomas. Seladin‐1 expression has been reported in the normal ovary. Granulosa cell tumours of the ovary (GCT) as with adrenocortical adenomas arise from a steroidogenic tissue, respond to pituitary hormone stimulation and synthesize steroid hormones. Design To test the hypothesis that seladin‐1 may also have a role in the pathogenesis of GCT, we determined the expression of seladin‐1 in a cohort of GCT and in mucinous and serous cystadenocarcinomas and in normal ovary. Measurements Expression was determined by RT‐PCR using gene specific primers and probes combined with Southern blot analysis of the PCR products. Results Seladin‐1 expression was identified in the normal ovary, mucinous cystadenocarcinomas and serous cystadenocarcinomas of the ovary whereas no expression was observed in the GCT. Conclusions Based on our results, seladin‐1 is not expressed in the granulosa cells or at least not in those that give rise to GCT.