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Glycosylated and non‐glycosylated prolactin forms are increased after opioid administration as part of surgical anaesthesia
Author(s) -
Priou A.,
Bruder N.,
Bégou D.,
Morange I.,
Grazianl N.,
Grisoll F.,
Brue T.
Publication year - 1995
Publication title -
clinical endocrinology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.055
H-Index - 147
eISSN - 1365-2265
pISSN - 0300-0664
DOI - 10.1111/j.1365-2265.1995.tb01917.x
Subject(s) - prolactin , medicine , endocrinology , basal (medicine) , stimulation , opioid , opiate , concomitant , hormone , insulin , receptor
Summary OBJECTIVE Previous studies have shown that non‐glycosylated prolactin (NG‐PRL) Increased more markedly than glycosylated hormone (G‐PRL) after TRH or metoclopramlde stimulation. The aim of the present study was to determine whether such results could be extended to oplold‐lnduced PRL stimulation. DESIGN Open and prospective study. Using a newly developed IRMA specific for NG‐PRL, we determined G‐PRL and NG‐PRL immunoreactivities after administration of 0.8‐1.2 mg of the opioid drug phenoperidlne as part of an anaesthesia. PATIENTS Ten male patients anaesthetized for surgical treatment of a prolapsed lumbar intervertebral disc. MEASUREMENTS Samples were obtained hourly pre and post‐operatively, and every 15 minutes during operation for determination of plasma PRL, NG‐PRL and G‐PRL. Plasma Cortisol, ACTH and GH levels were measured in an attempt to differentiate the respective roles of stress and opiate agonists In the variations of PRL levels during surgery. RESULTS A dramatic Increase in PRL levels was observed In all patients from an average of 300 ± 90 to 1200 ± 330 μ/l (mean + SEM) 30 minutes after drug administration. The proportion of G‐PRL immunoreactivity was not significantly different when basal (25‐2%) and stimulated (27%) values were compared ( P > 0.05), and when mean increments of NG‐PRL and G‐PRL were compared (345 and 348%, respectively). The opioid drug induced a significant decrease In Cortisol levels after injection and during operation (from 585 ± 63 to 99 ±51nmol/l) with a concomitant decrease In ACTH levels. GH levels were not significantly altered during anaesthesia but were significantly greater ( P < 0–05) after than before surgery (5–0 ±1.3 vs 0.98 ± 0.54 mU/l, respectively). CONCLUSIONS We conclude from the present and from previous data that opioid induced anaesthesia is accompanied by an increase In both glycosylated and non‐glycosylated PRL and that different PRL secreta‐gogues may Induce distinct responses In terms of PRL molecular forms.