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Effects of oestrogen on progesterone synthesis and arachidonic acid metabolism in human luteal cells
Author(s) -
Fisch B.,
Rose M. P.,
Elder M. G.,
Winston R. M. L.,
Margara R. A.,
Hillier S. G.
Publication year - 1994
Publication title -
clinical endocrinology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.055
H-Index - 147
eISSN - 1365-2265
pISSN - 0300-0664
DOI - 10.1111/j.1365-2265.1994.tb02439.x
Subject(s) - endocrinology , medicine , luteal phase , metabolism , estrogen , arachidonic acid , arachidonic acid metabolism , chemistry , progesterone receptor , biology , hormone , biochemistry , enzyme , estrogen receptor , cancer , breast cancer
Summary OBJECTIVE Locally produced oestrogens and prostaglandins (PGs) are implicated in the regulation of luteal lifespan in the human ovary. This study (1) assesses direct effects of these factors on progesterone synthesis in isolated luteal cells, and (2) explores interactions between luteal age and treatment with gonadotrophin or oestrogen on the metabolism of arachidonic acid (prostaglandin precursor) by steroidogenic luteal cells in vitro. DESIGN Primary monolayer cultures of human luteal cells obtained at different stages of the luteal phase were used to investigate the effect of oestradiol, catechol oestrogens (2‐ and 4‐hydroxyoestradiol), diethylstilboestrol, PGE 2 and PGF 2x on basal and human chorionic gonadotrophin (hCG) stimulated progesterone production in vitro. The role of PGs as modulators of luteal cell function was further investigated by studying the metabolic fate of radioactively labelled arachidonic acid in hormone treated (oestradiol and hCG) and control cultures, assessed by high performance liquid chromatography. ATIENTS Corpora lutea were enucleated from nine women with regular ovulatory cycles undergoing microsurgical reversal of tubal sterilization. Granulosa cell aspirates were obtained from three patients undergoing in‐vitro fertilization treatment. RESULTS PGE 2 and PGF 2α , at various concentrations did not have a consistent effect, whereas oestradiol, diethylstilboestrol (and 2‐hydroxyoestradiol in early luteal cell cultures) significantly inhibited basal and hCG stimulated progesterone biosynthesis. Evidence for direct inhibition of 3 β ‐hydroxysteroid dehydrogenase enzymic activity by oestradiol was obtained. Both major metabolic pathways of arachidonic acid (lipoxygenase and cyclo‐oxygenase) were operative in steroidogenic luteal cells recovered throughout the luteal phase. The ratio of PGE 2 to PGF 2 synthesis in vitro by human luteal cells from endogenously incorporated arachidonic acid did not change significantly with corpus luteum age, with PGE 2 tending to predominate. Oestradiol treatment shifted arachidonic acid metabolism from the lipoxygenase towards the cyclo‐oxygenase pathway in cells isolated from ageing corpora lutea. CONCLUSIONS Oestradiol, at relatively high concentrations, is a potent inhibitor of basal and hCG induced luteal cell steroidogenesis in vitro. No support is provided for the concept that luteolysis is mediated by local production of PGF 2α . The putative luteolytic effect of oestradiol may entail reduced metabolism of arachidonic acid to lipoxygenase derived products by luteal cells rather than direct stimulation of prostaglandin production by itself.