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HUMAN GRANULOSA CELLS CONTAIN INSULIN‐LIKE GROWTH FACTOR‐BINDING PROTEIN (IGF BP‐1) mRNA
Author(s) -
KOISTINEN RIITTA,
SUIKKARI ANNEMARIA,
TIITINEN AILA,
KONTULA KIMMO,
SEPPÄLÄ MARKKU
Publication year - 1990
Publication title -
clinical endocrinology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.055
H-Index - 147
eISSN - 1365-2265
pISSN - 0300-0664
DOI - 10.1111/j.1365-2265.1990.tb00907.x
Subject(s) - endocrinology , medicine , ovary , biology , insulin like growth factor binding protein , messenger rna , growth factor , decidua , northern blot , in situ hybridization , insulin like growth factor , dot blot , rna , fetus , gene , receptor , pregnancy , placenta , biochemistry , genetics
SUMMARY Previous studies have demonstrated expression of insulin‐like growth factor‐binding protein (IGF BP‐1) in secretory and decidualized endometrium, in adult and fetal liver, and in HepG2 liver cancer cells. We have studied the expression of IGF BP‐1 in various types of ovarian neoplasias, normal ovary, and granulosa cells from hyperstimulated human ovarian follicles by RNA blot hybridization. A single 1.6 kb mRNA species, similar to that present in human decidua, was identified in poly(A)RNA‐containing preparations of granulosa cells and of a borderline malignant ovarian cystadenoma. This finding verifies the postulated production of IGF BP‐1 by the human ovary.