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ANALYSIS OF HLA‐DQB AND HLA‐DPB ALLELES IN Graves'DISEASE BY OLIGONUCLEOTIDE PROBING OF ENZYMATICALLY AMPLIFIED DNA
Author(s) -
WEETMAN A. P.,
ZHANG LI,
WEBB SANDRA,
SHINE B.
Publication year - 1990
Publication title -
clinical endocrinology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.055
H-Index - 147
eISSN - 1365-2265
pISSN - 0300-0664
DOI - 10.1111/j.1365-2265.1990.tb00466.x
Subject(s) - allele , graves' disease , human leukocyte antigen , polymerase chain reaction , genomic dna , graves' ophthalmopathy , oligomer restriction , immunology , medicine , oligonucleotide , autoimmune disease , genotype , disease , biology , dna , genetics , antigen , gene
SUMMARY We have tested the possible association of HLA‐DQB and HLA‐DPB alleles with Graves'thyrotoxicosis, with or without severe ophthalmopathy, by polymerase chain amplification of genomic DNA and allele‐specific oligonucleotide probing. There was no significantly abnormal distribution of DQB alleles compared to 50 control subjects except for a reduced prevalence of DQw 3.1 in the Graves'patients with severe ophthalmopathy (x 2 = 6.23, P < 0.02). HLA‐DPB 2.1/8 was found in only 1 of 40 of these patients compared with 15 of the controls (x 2 = 11.49, P <0‐001). Ten of 48 patients with Graves'disease but without clinically significant eye involvement were HLA‐DPB 2.1/8 positive, not significantly different from controls, but significantly different from the ophthalmopathy group (x 2 = 6.70, P < 0.01). The other DPB alleles in both groups of Graves'disease patients were the same as controls. These results suggest that HLA‐DPB 2.1/8 may confer a protective effect in Graves'disease with respect to ophthalmopathy.