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EFFECT OF PORCINE BRAIN NATRIURETIC PEPTIDE (pBNP) ON HUMAN ADRENOCORTICAL STEROIDOGENESIS
Author(s) -
HASHIGUCHI TAKASHI,
HIGUCHI KAZUMI,
OHASHI MASAO,
TAKAYANAGI RYOICHI,
MATSUO HISAYUKI,
NAWATA HAJIME
Publication year - 1989
Publication title -
clinical endocrinology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.055
H-Index - 147
eISSN - 1365-2265
pISSN - 0300-0664
DOI - 10.1111/j.1365-2265.1989.tb01287.x
Subject(s) - medicine , endocrinology , receptor , brain natriuretic peptide , natriuretic peptide , aldosterone , peptide , adrenal cortex , biology , intracellular , chemistry , microbiology and biotechnology , biochemistry , heart failure
SUMMARY Porcine brain natriuretic peptide (pBNP), purified from porcine brain, had a significant suppressive effect on aldosterone and cortisol secretions in ACTH‐treated cultured human adrenal cells. Concomitantly, the intracellular cGMP formation was enhanced by pBNP treatment. A specific pBNP receptor was identified in the human adrenal tissues. Affinity labelling of 125 I‐BNP showed two separate molecular weights of specific binding sites for pBNP of 140 and 67 kDa. A 125 I‐BNP binding study of the human adrenal membrane fraction demonstrated the presence of high‐affinity and low‐capacity binding sites for pBNP. Moreover, these binding sites for 125 I‐pBNP were displaced by unlabelled α‐rANP as well as pBNP. From these studies, we concluded that pBNP had suppressive effects on human adrenocortical steroidogensis, possibly via a receptor which may be shared with ANP.