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CORTICOTROPHIN‐RELEASING FACTOR‐LIKE IMMUNOREACTIVITY IN HUMAN 24 H URINE
Author(s) -
MASERGLUTH CHRISTIANE,
VECSEI PAUL
Publication year - 1989
Publication title -
clinical endocrinology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.055
H-Index - 147
eISSN - 1365-2265
pISSN - 0300-0664
DOI - 10.1111/j.1365-2265.1989.tb00439.x
Subject(s) - endocrinology , medicine , urine , chemistry
SUMMARY This report is the first demonstration of the presence of corticotrophin‐releasing factor‐like immunoreactive material (CRF‐LI) in human urine. Our studies, using immunoaffinity chromatography for extraction, followed by reverse phase high performance liquid chromatography for further purification, and radioimmunoassay for final peptide measurement, suggest that CRF‐LI from human urine is similar, if not identical, to human CRF. Urinary CRF‐LI excretion in normal subjects was 1.48 ± 0.69 ng/24 h (mean ± SD; n = 20) with no significant differences between the sexes. During pregnancy, urinary CRF‐LI excretion increased, and a significant correlation between urinary CRF‐LI excretion and the gestational age was seen ( r = 0.62; P <0.0001). We suggest that urinary 24‐h CRF‐LI excretion may reflect the secretion of CRF into the circulation. Measurements of CRF‐LI in the urine may have several advantages over determinations of hormone levels in plasma.