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ANALYSIS OF TSH RECEPTORS AND MICROSOMAL ANTIGEN IN DIFFERENT HUMAN THYROID TISSUE SPECIMENS
Author(s) -
FURMANIAK J.,
DAVENPORT S.,
NAKAJIMA Y.,
KAISER U.,
MULLERGARTNER H.W.,
BUCKMANN F. W.,
PEGG C.,
SMITH B. REES
Publication year - 1988
Publication title -
clinical endocrinology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.055
H-Index - 147
eISSN - 1365-2265
pISSN - 0300-0664
DOI - 10.1111/j.1365-2265.1988.tb03850.x
Subject(s) - thyroid , thyroid carcinoma , endocrinology , medullary carcinoma , medicine , receptor , antigen , pathology , thyroid nodules , medullary cavity , microsome , chemistry , biology , biochemistry , enzyme , immunology
SUMMARY Affinity labelling with a 125 I‐labelled photoactive derivative of TSH (HSAB‐TSH) was used to analyse TSH receptor size in the following specimens of human thyroid tissue: (1) cold nodules; (2) autonomous nodules; (3) papillary carcinoma; (4) medullary carcinoma; (5) metastasis of papillary carcinoma to lymph node; (6) anaplastic carcinoma, and (7) Graves' thyroid. In addition, a sample of histologically normal thyroid tissue surrounding specimens 1–4 was analysed in each case. Thyroid microsomes were also prepared from the tissue samples, solubilized using 1% deoxycholate and labelled with 125 I. The preparations were immunoprecipitated using microscomal autoantibodies and protein A and analysed by SDS‐PAGE and autoradiography. These studies indicated that no differences in the characteristics of the TSH receptor or of microsomal antigen were observed in the tissue samples 1–3 and 7. Neither protein was detected in tissue specimens 4–6.