Premium
SECRETION OF INTACT PTH BY DISPERSED HUMAN HYPERPARATHYROID CELLS
Author(s) -
ASTON J. P.,
BROWN R. C.,
CURLEY I.,
WHEELER M. H.,
WOODHEAD J. S.
Publication year - 1988
Publication title -
clinical endocrinology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.055
H-Index - 147
eISSN - 1365-2265
pISSN - 0300-0664
DOI - 10.1111/j.1365-2265.1988.tb03712.x
Subject(s) - endocrinology , medicine , secretion , calcium , parathyroid chief cell , secretion rate , parathyroid hormone , hyperparathyroidism , chemistry , biology
SUMMARY The rate of secretion of intact PTH by cells dispersed from adenomatous and hyperplastic human parathyroid tissue was measured using a two‐site immuno‐chemiluminometric assay specific for the intact peptide. Secretion rates were monitored following incubation in high and low‐calcium media. The mean rate of secretion from hyperplastic cells was significantly greater ( P <005) at low calcium and significantly more suppressible at high calcium ( P <001) compared with adenomatous cells. These studies have demonstrated: (1) the calcium‐dependent secretion of intact PTH, (2) that intact hormone release contributes to the non‐suppressible component of secretion, and (3) differences in the secretory characteristics of adenomatous and hyperplastic parathyroid cells.