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DETERMINATION OF ENOLASE ISOZYMES IN VARIOUS ADRENAL GLAND TUMOURS
Author(s) -
YONEDA M.,
TAKATSUKI K.,
YAMAUCHI K.,
OISO Y.,
TOMITA A.,
MIZUNO S.,
KATO K.
Publication year - 1987
Publication title -
clinical endocrinology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.055
H-Index - 147
eISSN - 1365-2265
pISSN - 0300-0664
DOI - 10.1111/j.1365-2265.1987.tb00787.x
Subject(s) - enolase , primary aldosteronism , endocrinology , medicine , radioimmunoassay , adenoma , isozyme , immunoassay , pheochromocytoma , adrenal gland , chemistry , enzyme , antibody , immunohistochemistry , aldosterone , biochemistry , immunology
SUMMARY Enolase isozymes (α enolase and γ enolase) in the extracts of adrenal tumours (phaeochromocytoma, adenoma of primary aldosteronism and Cushing's syndrome, and neurinoma) were determined by means of enzyme immunoassay systems. The mean ± SEM, respectively, of α and γ enolase levels were 2.5 ± 0.37 μg/mg protein and 3‐2 ± 0–69 μg/mg protein for 9 phaeochromocytomas, 15.2 + 3.1 μg/mg protein and 0.65 ± 0.18 μg/mg protein for three adenomas with primary aldosteronism, 10.8 ± 3.0 μg/mg protein and 0.23 ± 0.02 μg/mg protein for five adenomas causing Cushing's syndrome, and 3.8 ± 0.88 μg/mg protein and 0.30 ± 0.15 μg/mg protein for three neurinomas. Thus, the γ enolase concentration in the extract of phaeochromocytoma was higher than that of other adrenal tumours. The serum level of γ enolase was determined in 36 patients with adrenal tumours and 26 normal controls by radioimmunoassay. The mean + SEM of γ enolase level was 5.4 ± 0.3 ng/ml in normal controls, 91 ± 0.9 ng/ml for 10 patients with phaeochromocytoma, 6.3 ± 0.3 ng/ml for 11 with primary aldosteronism, 5.5 ± 0.4 ng/ml for 11 with Cushing's syndrome, and 5.1 ± 0.7 ng/ml for four with neurinoma. Thus, patients with phaeochromocytoma had a significantly higher serum γ enolase levels than did those with tumours derived from adrenal cortex and normal controls. In patients with phaeochromocytoma, serum γ enolase levels showed a significant positive correlation with urinary adrenaline levels ( P <0.05), and after resection the elevated level of γ enolase fell significantly ( P <0.05) and returned to normal. These results indicate that γ enolase in serum may be a useful marker for the differential diagnosis of adrenal tumours and therapeutic monitoring of phaeochromocytoma.