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HIGH MOLECULAR WEIGHT VASOPRESSIN: DETECTION OF A LARGE AMOUNT IN THE PLASMA OF A PATIENT
Author(s) -
SMITZ S.,
LEGROS J.J.,
FRANCHIMONT P.,
MAIRE M.
Publication year - 1985
Publication title -
clinical endocrinology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.055
H-Index - 147
eISSN - 1365-2265
pISSN - 0300-0664
DOI - 10.1111/j.1365-2265.1985.tb01095.x
Subject(s) - vasopressin , sephadex , antidiuretic , medicine , radioimmunoassay , endocrinology , arginine , trypsin , incubation , chemistry , urea , chromatography , syndrome of inappropriate antidiuretic hormone secretion , biochemistry , biology , amino acid , enzyme
SUMMARY Using a sensitive and specific radioimmunoassay for arginine‐vasopressin, we have searched for the presence of high molecular weight (HMW) vasopressin in the plasma of a patient with the syndrome of inappropriate antidiuretic hormone secretion (SIADH) and an oat cell carcinoma of the lung. After incubation in 8 mol/l urea, one millilitre of the plasma from this patient was fractionated on a Sephadex G‐50 column and the immunoreactive vasopressin content was evaluated before and after trypsin treatment of the eluted fractions. A wide peak of apparent mol. wt. 2500–6000 daltons was revealed only after tryptic digestion of each fraction. This peak contained the equivalent of 1900 pg of vasopressin. A tryptic digest of this peak, rechromatographed on Sephadex G‐25, gave two small peptides the major one eluting at a position identical to vasopressin. These results demonstrate the presence of a large amount of HMW vasopressin in the plasma of a patient with SIADH and oat cell carcinoma of the lung.