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THE INVOLVEMENT OF THE PENTOSE SHUNT IN THYROID METABOLISM AFTER STIMULATION WITH TSH OR WITH IMMUNOGLOBULINS FROM PATIENTS WITH THYROID DISEASE. II. THE REOXIDATION OF NADPH AND STIMULATION OF HORMONE SYNTHESIS
Author(s) -
DREXHAGE H. A.,
HAMMOND LINDA J.,
BITENSKY LUCILLE,
CHAYEN J.,
BOTTAZZO G. F.,
DONIACH DEBORAH
Publication year - 1982
Publication title -
clinical endocrinology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.055
H-Index - 147
eISSN - 1365-2265
pISSN - 0300-0664
DOI - 10.1111/j.1365-2265.1982.tb03147.x
Subject(s) - organification , medicine , endocrinology , thyroid , stimulation , hormone , iodine , pentose phosphate pathway , in vitro , chemistry , guinea pig , antibody , metabolism , biology , biochemistry , immunology , glycolysis , organic chemistry
SUMMARY Reducing equivalents derived from the tissue re‐oxidation of NADPH (NADPH‐diaphorase) have been implicated in the peroxidation that is involved in the organification of iodine in the production of thyroid hormones. Immunoglobulin (Ig) fractions from patients with thyroid diseases and from normal controls, in a standard dose of 125 μg/ml and 0±3 μ/ml thyrotrophin (TSH) were incubated with segments of guinea‐pig thyroid gland maintained in vitro. A quantitative cytochemical study was made on how these fractions influenced the enzyme activity. A good correlation was found between the ability of such Ig fractions to stimulate the NADPH‐diaphorase activity and (1) the degree of hyperthyroidism in the patients and (2) the amount of T3 secreted by the thyroid segments in vitro.