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DEMONSTRATION OF CIRCULATING 1,24,25‐TRIHYDROXYVITAMIN D 3 IN MAN BY RADIOIMMUNOASSAY
Author(s) -
CLEMENS T. L.,
FRAHER L. J.,
SANDLER L. M.,
O'RIORDAN J. L. H.
Publication year - 1982
Publication title -
clinical endocrinology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.055
H-Index - 147
eISSN - 1365-2265
pISSN - 0300-0664
DOI - 10.1111/j.1365-2265.1982.tb00725.x
Subject(s) - radioimmunoassay , medicine , endocrinology , chemistry
SUMMARY 1,24,25‐trihydroxyvitamin D 3 has been detected in human serum using a sensitive radioimmunoassay. Tritiated 1,24,25‐trihydroxyvitamin D 3 was synthesized biologically and used as tracer to monitor the recovery of endogenous metabolite during isolation from serum. Circulating 1,24,25(OH) 3 D 3 in normal subjects ranged from 9·3 to 18·5 pmol/l but was not detectable (> 2·3 pmol/l) in serum from nephrectomized subjects. The trihydroxy metabolite was elevated in three out of four vitamin D deficient subjects who were being treated with vitamin D 3 .