Premium
MEASUREMENT OF 1,25‐DIHYDROXYCHOLECALCIFEROL IN MAN BY RADIOIMMUNOASSAY
Author(s) -
CLEMENS T. L.,
HENDY G. N.,
PAPAPOULOS S. E.,
FRAHER L. J.,
CARE A. D.,
O'RIORDAN J. L. H.
Publication year - 1979
Publication title -
clinical endocrinology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.055
H-Index - 147
eISSN - 1365-2265
pISSN - 0300-0664
DOI - 10.1111/j.1365-2265.1979.tb03068.x
Subject(s) - radioimmunoassay , antiserum , sephadex , medicine , endocrinology , bovine serum albumin , titer , chemistry , serum albumin , chromatography , albumin , vitamin d and neurology , chronic renal failure , high performance liquid chromatography , antibody , biochemistry , immunology , enzyme
SUMMARY 1α,25‐dihydroxycholecalciferol (1,25‐(OH) 2 D 3 ) has been measured in human serum by radioimmunoassay. The assay uses a high titre antiserum raised in sheep against 1,25‐(OH) 2 D 3 ‐25‐hemisuccinate, conjugated to bovine serum albumin. The sensitivity of the assay is 10 pg/tube. Other hydroxylated forms of vitamin D 3 cross react with the antiserum and are therefore removed from serum extracts by chromatography on Sephadex LH 20 followed by high pressure liquid chromatography. The mean (± SEM) serum 1,25‐(OH) 2 D 3 concentration for a group of healthy adult subjects was 41 ± 2.5 pg/ml. None was detected in anephric patients and the concentration was low or undetectable in patients with chronic renal failure.