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SCREENING FOR NEONATAL HYPOTHYROIDISM BY THYROXINE AND THYROTROPHIN RADIOIMMUNOASSAYS USING DRIED BLOOD SAMPLES ON FILTER PAPER
Author(s) -
BECKERS C.,
CORNETTE C.,
FRANCOIS B.,
BOUCKAERT A.
Publication year - 1979
Publication title -
clinical endocrinology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.055
H-Index - 147
eISSN - 1365-2265
pISSN - 0300-0664
DOI - 10.1111/j.1365-2265.1979.tb02116.x
Subject(s) - radioimmunoassay , endocrinology , medicine , dried blood , congenital hypothyroidism , chemistry , chromatography , thyroid
SUMMARY A routine and automatized methodology for thyroxine (T4) and thyrotrophin (TSH) radioimmunoassay (RIA) using dried blood samples on filter paper is described. T4‐RIA was performed on one single dot (5 mm diameter equivalent to 4 μl of serum) while two dots were necessary for TSH‐RIA. Reference filter papers were introduced in each assay for quality control. In a preliminary study on 4,155 neonates, samples generally obtained between the 5th–7th day gave a mean ‘dot‐T4′ of 97.95 ± 36.04 nmol/l and a mean ‘dot‐TSH’ of 10.19 mU/l ±8.25, corresponding to 2.47 mU/l of serum. Within an 18‐month period (November 1976‐April 1978), a total of 16.522 neonates have been screened allowing detection of three cases of congenital hypothyroidism (incidence 1: 5507), two cases of congenitally low TBG and thirty‐three cases of transient hypothyroidism.