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THE CHARACTERIZATION OF GROWTH HORMONE RELEASE INHIBITING HORMONE‐LIKE IMMUNOREACTIVITY IN NORMAL URINE
Author(s) -
KRONHEIM S.,
BERELOWITZ M.,
PIMSTONE B. L.
Publication year - 1977
Publication title -
clinical endocrinology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.055
H-Index - 147
eISSN - 1365-2265
pISSN - 0300-0664
DOI - 10.1111/j.1365-2265.1977.tb01332.x
Subject(s) - sephadex , chemistry , urine , radioimmunoassay , endocrinology , medicine , elution , chromatography , immunoassay , sepharose , hormone , acromegaly , growth hormone , antibody , biochemistry , biology , immunology , enzyme
SUMMARY. Using a previously described radioimmunoassay for growth hormone release inhibiting hormone (GH‐RIH), the presence of GH‐RIH‐like immunoreactivity in urine has been characterized by demonstrating mobility identical to synthetic GH‐RIH standard on two sephadex gel chromatographic systems, and parallelism of dilutions of the sephadex fractions with synthetic GH‐RIH. Furthermore, 74% of the sephadex fraction cross‐reacting in the immunoassay bound to antibody conjugated to sepharose and could be eluted by 1 M acetic acid. This immunospecific eluate showed identity with synthetic GH‐RIH on both ion exchange and thin layer chromatography. Thus GH‐RIH‐like immunoreactivity is present in normal urine; this may have potential relevance in the search for a physiological role for this peptide.