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THE CHEMISTRY AND CONFORMATIONAL AND BIOLOGICAL ANALYSIS OF VITAMIN D 3 , ITS METABOLITES AND ANALOGUES
Author(s) -
NORMAN ANTHONY W.,
JOHNSON R. LORNE,
OSBORN TERRY W.,
PROSAL DANA A.,
CAREY STEPHEN C.,
HAMMOND MILTON L.,
MITRA MANINDARAN N.,
PIRIO MARCEL R.,
REGO ALBERT,
WING RICHARD M.,
OKAMURA WILLIAM H.
Publication year - 1976
Publication title -
clinical endocrinology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.055
H-Index - 147
eISSN - 1365-2265
pISSN - 0300-0664
DOI - 10.1111/j.1365-2265.1976.tb03821.x
Subject(s) - chemistry , vitamin d and neurology , cytosol , biological activity , in vivo , calcium , vitamin , steroid , nuclear magnetic resonance spectroscopy , biochemistry , stereochemistry , endocrinology , in vitro , medicine , steroid hormone , receptor , hormone , biology , enzyme , organic chemistry , microbiology and biotechnology
SUMMARY The chemical properties, sterochemical relationships and solution confromation, as assessed in part by proton NMR spectroscopy, for vitamin D 3 , its major metabolites [including 1α, 25‐(OH) 2 D 3 , its hormonally active form] and a number of A‐ring and side chain analogues are evaluated and discussed in relation ot their biological activity. In particular the relative ability of many of these seco‐steroids to compet both with 25‐OHD 3 for its chick serum binding protein and 1α, 25‐(OH) 2 ‐D 3 for its chick intenstinal cytosol‐chromation receptor systme was quantitated, in vitro , Further, the relative effectiveness of all these metabolites and analogues to mediate in vivo intestinal calcium absorption and bone calcium mobilization was determined. Collectiveily these chemical and bilogical studies constitute a ‘systems analysis’of the various steroid structural parameters both required and tolerated by the multi‐stepped endocrine system associated with the bilogical actions of vitamin D.