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CLEARANCE AND IDENTIFICATION OF THYROTROPHIN RELEASING HORMONE IN HUMAN URINE AFTER INTRAVENOUS INJECTIONS
Author(s) -
JEFFCOATE S. L.,
WHITE NICKI
Publication year - 1975
Publication title -
clinical endocrinology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.055
H-Index - 147
eISSN - 1365-2265
pISSN - 0300-0664
DOI - 10.1111/j.1365-2265.1975.tb01549.x
Subject(s) - urine , radioimmunoassay , endocrinology , tripeptide , medicine , chemistry , basal (medicine) , hormone , thyrotropin releasing hormone , peptide , chromatography , biochemistry , insulin
SUMMARY The urine clearance of TRH after intravenous injection in man has been measured by radioimmunoassay. Between 4.4% and 10.7% of the dose was excreted within 90 min, the majority within 30 min. The TRH excreted was immunochemically and chromatographically indistiguishable from synthetic TRH and was inactivated by plasma enzymes with the same kinetic characteristics. The immunoreactive TRH‐like material in basal urine samples was not TRH however: chromato‐graphically and enzymatically it behaved differently from the synthetic tripeptide.