Premium
DISAPPEARANCE OF LH‐RELEASING HORMONE IN MAN AS DETERMINED BY RADIOIMMUNOASSAY
Author(s) -
ARIMURA AKIRA,
KASTIN ABBA J.,
GONZALEZBARCENA D.,
SILLER JORGE,
WEAVER ROBERT E.,
SCHALLY ANDREW V.
Publication year - 1974
Publication title -
clinical endocrinology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.055
H-Index - 147
eISSN - 1365-2265
pISSN - 0300-0664
DOI - 10.1111/j.1365-2265.1974.tb02812.x
Subject(s) - radioimmunoassay , endocrinology , medicine , inulin , luteinizing hormone , chemistry , hormone , excretion , extracellular , biology , biochemistry
The disappearance of LH‐releasing hormone (LH‐RH) administered i.v. together with inulin was measured by radioimmunoassay. The half‐time disappearance of 3.6 min for LH‐RH was significantly shorter than the half‐time of 14.2 min found for the inulin. On the assumption that diffusion and distribution into the extracellular space and excretion are quantitatively similar for LH‐RH and inulin, the part of the half‐life of LH‐RH due to metabolic breakdown can be calculated to be about 4.8 min. Peak LH and FSH values were found to occur later. The results suggest that most of the immunoreactive LH‐RH is no longer present in the blood at the time of maximal gonadotropin release and that this is because of rapid metabolism as well as diffusion and excretion.