Premium
PLASMA CORTICOTROPHIN LEVELS DURING INSULIN‐HYPOGLYCAEMIA : COMPARISON OF RADIOIMMUNOASSAY AND CYTOCHEMICAL BIOASSAY
Author(s) -
FLEISHER M. R.,
GLASS D.,
BITENSKY LUCILLE,
CHAYEN J.,
DALY J. R.
Publication year - 1974
Publication title -
clinical endocrinology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.055
H-Index - 147
eISSN - 1365-2265
pISSN - 0300-0664
DOI - 10.1111/j.1365-2265.1974.tb01796.x
Subject(s) - radioimmunoassay , bioassay , endocrinology , medicine , insulin , adrenocorticotropic hormone , chemistry , biology , hormone , genetics
SUMMARY Development of a highly sensitive bioassay for ACTH has enabled bioassayable ACTH to be measured in plasma during the course of the insulin hypoglycaemia test for the first time. Comparison with radioimmunoassay (RIA) on the same plasma samples shows that the latter consistently overstimates the true, or biologically active, ACTH level. This overstimate appears to be most marked when ACTH levels are highest and during the fall in plasma levels which occurs following the ACTH peak. This may limit the value of the RIA of ACTH, in some situations.